Journal:

Article Title: Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord

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Figure Lengend Snippet: Tissue distribution of human prepro-UII mRNA. (A) Dot blot analysis of prepro-UII mRNA expression in various human tissues. The master blot (CLONTECH) consisted of 50 human-tissue poly(A) RNAs (80–448 ng per dot) normalized by using the RNA expression levels of eight housekeeping genes. Positive controls consisted of human genomic DNA. Negative controls included yeast and Escherichia coli RNA or DNA, as well as human repetitive genomic sequences. The blot was probed with the human prepro-UII cDNA probe and exposed for 2 days onto an X-Omat film (B) Northern blot analysis of prepro-UII mRNA expression in the human spinal cord. Spinal cord poly(A) mRNA (2 μg) was hybridized with the human prepro-UII cDNA probe. The molecular weight was determined by using RNA markers. (C) X-ray autoradiographs showing the distribution of prepro-UII mRNA in the human spinal cord. Coronal sections were hybridized with the antisense (Top) or sense (Bottom) prepro-UII riboprobe and exposed for 10 days onto x-ray film.

Article Snippet: Thus, human spinal cord poly(A) RNA (CLONTECH) was used for amplification of the 5′-end of human UII cDNA with a commercially available rapid amplification of cDNA ends (RACE) kit (Marathon cDNA amplification kit, CLONTECH).

Techniques: Dot Blot, Expressing, RNA Expression, Genomic Sequencing, Northern Blot, Molecular Weight